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Merck

E7889

Ethylenediaminetetraacetic acid disodium salt solution

Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

동의어(들):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS 번호:
139-33-3
Molecular Weight:
336.21
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
24894660
MDL number:
MFCD00070672
Beilstein/REAXYS Number:
3822669

주요 문서

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도움 문의

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

Quality Level

300

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관련 카테고리

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H373

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number
SLCL5409
BCCG6590
BCCG1836
BCCG0945
BCCF9230

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Jarrod S Johnson et al.
Cell host & microbe, 23(3), 366-381 (2018-03-16)
Myeloid dendritic cells (DCs) have the innate capacity to sense pathogens and orchestrate immune responses. However, DCs do not mount efficient immune responses to HIV-1, primarily due to restriction of virus reverse transcription, which prevents accumulation of viral cDNA and
Yongchao Dou et al.
Cell, 180(4), 729-748 (2020-02-16)
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Joshua B Black et al.
Cell reports, 33(9), 108460-108460 (2020-12-03)
Technologies to reprogram cell-type specification have revolutionized the fields of regenerative medicine and disease modeling. Currently, the selection of fate-determining factors for cell reprogramming applications is typically a laborious and low-throughput process. Therefore, we use high-throughput pooled CRISPR activation (CRISPRa)
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