E9379
Stains-All
~95% purity, powder
동의어(들):
1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
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제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C30H27BrN2S2
CAS 번호:
Molecular Weight:
559.58
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
231-047-7
MDL number:
Beilstein/REAXYS Number:
3865213
제품 이름
Stains-All, ~95%
assay
~95%
Quality Level
form
powder
technique(s)
microbe id | staining: suitable
solubility
chloroform: 40 mg (PLUS 4.0 ml)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
[Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
InChI
1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
InChI key
MPBRYMWMMKKRGC-UHFFFAOYSA-M
Application
Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.
Biochem/physiol Actions
Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).
As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.
As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.
Disclaimer
Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Rehm H
Protein Biochemistry and Proteomics. The experimenter series (2006)
Chromatin and Chromosomal Protein Research III (1978)
Kinetics of duplex formation for individual DNA strands within a single protein nanopore.
Howorka S, et al.
Proceedings of the National Academy of Sciences of the USA, 98, 12996-12996 (2001)