M8884

Methyl Green

Name: Methyl Green
Brand: SIAL

~85% purity, powder

동의어(들):

Ethyl Green

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

C₂₇H₃₅BrClN₃ · ZnCl₂

CAS 번호:

7114-03-6

Molecular Weight:

653.24

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24897293

EC Number:

230-415-4

MDL number:

MFCD00151094

Colour Index Number:

42590

Beilstein/REAXYS Number:

3914106

주요 문서

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기술 서비스

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도움 문의

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제품 이름

Methyl Green, zinc chloride salt, ~85%

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

assay

~85%

form

powder

composition

Dye Content, ≥83%

technique(s)

titration: suitable

color

red to very dark red

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 1 mg/mL

ε_max

>11100.00 at 417-425 nm in water at 0.005 g/L, >16000 at 311-319 nm in water at 0.005 g/L, >60000 at 626-634 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Application

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.