N6876

Nitrotetrazolium Blue chloride

Name: Nitrotetrazolium Blue chloride
Brand: SIAL

≥90.0% (HPLC)

동의어(들):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

C₄₀H₃₀N₁₀O₆ · 2Cl

CAS 번호:

298-83-9

Molecular Weight:

817.64

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24897795

MDL number:

MFCD00012159

Beilstein/REAXYS Number:

4115923

주요 문서

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기술 서비스

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도움 문의

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InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H₂O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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General description

Substrate for dehydrogenases and other oxidases.

Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.