P8341
TWEEN® 20
Low-peroxide; Low-carbonyls
동의어(들):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
description
non-ionic
form
viscous liquid
Quality Level
impurities
≤0.5 μmol/g Peroxides, ≤1.0 μmol/g Carbonyls, ≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
transition temp
cloud point 76 °C
density
1.1 g/cm3 at 25 °C (lit.)
HLB
16.7
storage temp.
−20°C
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General description
Tween®20, is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
Non-ionic detergent
TWEEN 20 is widely used in several biochemical applications. It has been used:
- As an emulsifying agent for the preparation of stable oil-in-water emulsions
- In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
- As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
- For lysing mammalian cells at a concentration of 0.005 to 0.5%
Other Notes
Preservative Free
Legal Information
TWEEN is a registered trademark of Croda International PLC
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
Martindale: The Extra Pharmacopoeia, 1030-1030 null
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Determination of telomere length is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Fluorescence in situ hybridization (FISH) of telomere repeats has been used to calculate telomere
Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
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