InChI
1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
InChI key
BNIILDVGGAEEIG-UHFFFAOYSA-L
product line
BioReagent, Redi-Dri™
assay
≥99.0%
form
powder or crystals
quality
free-flowing
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
pH
8.9-9.2 (25 °C, 50 g/L in water)
pKa (25 °C)
(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
solubility
water: soluble 100 mg/mL
application(s)
diagnostic assay manufacturing
Quality Level
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General description
Sodium phosphate dibasic is a component in many biological buffers used in biochemical, biomedical, and molecular biological research. It is also known as disodium hydrogen phosphate (Na2HPO4), it is an inorganic salt that is highly soluble in water. It serves as a versatile buffering agent in research with a pH of 8.9 - 9.2. It is essential for making several phosphate buffers including PBS, utilized for cell culture, immunohistochemistry, Western blot, ELISA, and other applications where a stable pH is critical.
Application
Sodium phosphate dibasic may be used for phosphate buffer and Phosphate Buffered Saline (PBS) that is commonly utilized for cell culture, immunohistochemistry, western blot, and other biochemical applications where a stable pH is crucial. Including protein purification and enzymatic assays.
Features and Benefits
- Qualified for use in cell culture; suitable for insect cell culture
- Redi-Dri™ packaging controls moisture for hygroscopic chemicals that commonly clump
- Anhydrous powder remains free-flowing and easy to use
- Safer to handle and weigh out for routine use in lab
- Eliminates clumps, maintaining product moisture, quality, and attributes
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
저장 등급
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Evaluation of Burkholderia cepacia complex bacteria pathogenicity using Caenorhabditis elegans
Tedesco P, et al.
Bio-protocol, 6(20) (2016)
Isolating lipid droplets from multiple species
Ding Y, et al.
Nature Protocols, 8(1), 43-43 (2013)
Sayed-Hadi Mirmalek-Sani et al.
Biomaterials, 34(22), 5488-5495 (2013-04-16)
Emergent technologies of regenerative medicine have the potential to overcome the limitations of organ transplantation by supplying tissues and organs bioengineered in the laboratory. Pancreas bioengineering requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native
Kaisorn L Chaichana et al.
Journal of neuroscience methods, 180(1), 116-125 (2009-05-12)
The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such
C Tony Liu et al.
Journal of the American Chemical Society, 136(29), 10349-10360 (2014-07-01)
Electrostatic interactions play an important role in enzyme catalysis by guiding ligand binding and facilitating chemical reactions. These electrostatic interactions are modulated by conformational changes occurring over the catalytic cycle. Herein, the changes in active site electrostatic microenvironments are examined
문서
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
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