08445
7-Amino-4-methyl-3-coumarinylacetic acid
BioReagent, suitable for fluorescence, ~90% (HPLC)
동의어(들):
2H-1-Benzopyran-3-acetic acid, AMCA-H, Aminomethyl coumarin acetic acid
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제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C12H11NO4
CAS 번호:
Molecular Weight:
233.22
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352106
MDL number:
Beilstein/REAXYS Number:
7927205
product line
BioReagent
Quality Segment
assay
~90% (HPLC)
form
powder
solubility
DMF: soluble, DMSO: soluble, aqueous base: soluble
fluorescence
λex 350 nm; λem 433 nm in methanol
suitability
suitable for fluorescence
InChI
1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
InChI key
QEQDLKUMPUDNPG-UHFFFAOYSA-N
Application
AMCA is a useful agent for labeling proteins. Demonstrates a characteristic absorption peak at 345 nm with a fluorescence emission at 440-460 nm (blue region); however, when conjugated with proteins the absorption peak shifts to 355 nm with a fluorescence emission at 440-460 nm. It has a high fluorescence yield with a sufficient Stokes shift (100 nm) and is relatively photostable . Relatively easy to activate and couple to the N-terminal amino group of a peptide resulting in an acid stable amide bond .
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Useful blue fluorophore for immunofluorescence, λabs ~350 nm, λem ~440 nm
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
P A Muraro et al.
The Journal of clinical investigation, 100(2), 339-349 (1997-07-15)
The pathogenesis of multiple sclerosis (MS) is currently ascribed in part to a T cell-mediated process targeting myelin components. The T cell response to one candidate autoantigen, myelin basic protein (MBP), in the context of HLA-DR15Dw2, has been previously studied
D Delia et al.
Cytometry, 12(6), 537-544 (1991-01-01)
Antibodies coupled to 7-aminocoumarin (AMCA) emit a bright blue fluorescence under ultraviolet (UV) excitation and are therefore ideal for three-color immunofluorescence (IF) with fluorescein (FITC) and phycoerythrin (PE) labeled reagents; however, due to the different absorption spectra, the use of
H Khalfan et al.
The Histochemical journal, 18(9), 497-499 (1986-09-01)
A new fluorescent protein labelling agent, 7-amino-4-methyl coumarin-3-acetic acid (AMCA), emits in the blue region (440-460 nm) on activation with UV light (350 nm). The active reagent is the N-hydroxysuccinimide ester which reacts with lysine residues under mild conditions to