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Merck

94403

Hoechst 33258 solution

1 mg/mL in H2O, ≥98.0% (HPLC)

동의어(들):

bisBenzimide H 33258 solution

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C25H24N6O · 3HCl
CAS 번호:
23491-45-4
Molecular Weight:
533.88
MDL number:
MFCD29074011
UNSPSC Code:
12171500
PubChem Substance ID:
329770334
NACRES:
NA.32
Assay:
≥98.0% (HPLC)
Form:
liquid
Quality level:
100

주요 문서

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SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

assay

≥98.0% (HPLC)

form

liquid

Quality Level

100

mol wt

533.88 g/mol

concentration

1 mg/mL in H2O

color

colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble, H2O: soluble

density

1.00 g/cm3

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

storage temp.

2-8°C

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCK6782
BCCH3038
BCCH1899
BCCG8111
BCCG4764

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리 방문

Cornelia Fritsch et al.
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Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
Rajam S Mani et al.
The Journal of biological chemistry, 294(2), 520-530 (2018-11-18)
Polynucleotide kinase/phosphatase (PNKP) and X-ray repair cross-complementing 1 (XRCC1) are key proteins in the single-strand DNA break repair pathway. Phosphorylated XRCC1 stimulates PNKP by binding to its forkhead-associated (FHA) domain, whereas nonphosphorylated XRCC1 stimulates PNKP by interacting with the PNKP
Hansruedi Mathys et al.
Nature, 570(7761), 332-337 (2019-05-03)
Alzheimer's disease is a pervasive neurodegenerative disorder, the molecular complexity of which remains poorly understood. Here, we analysed 80,660 single-nucleus transcriptomes from the prefrontal cortex of 48 individuals with varying degrees of Alzheimer's disease pathology. Across six major brain cell
Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
Ismail Abdou et al.
Nucleic acids research, 43(2), 875-892 (2014-12-30)
In the current model of DNA SSBR, PARP1 is regarded as the sensor of single-strand breaks (SSBs). However, biochemical studies have implicated LIG3 as another possible SSB sensor. Using a laser micro-irradiation protocol that predominantly generates SSBs, we were able
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