A2220
ANTI-FLAG® M2 Affinity Gel
purified immunoglobulin, buffered aqueous glycerol solution
동의어(들):
Monoclonal ANTI-FLAG® M2, ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk
크기 선택
제품정보 (DICE 배송 시 비용 별도)
conjugate
agarose conjugate
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
(4% agarose bead; 45-165μm bead size)
isotype
IgG1
capacity
>0.6 mg/mL, resin binding capacity (FLAG-BAP)
shipped in
wet ice
storage temp.
−20°C
General description
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Immunogen
Application
Learn more product details in our FLAG® application portal.
Physical form
Legal Information
Disclaimer
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저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
문서
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
관련 콘텐츠
이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합형 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.