A4596
ANTI-FLAG® M1 Agarose Affinity Gel
동의어(들):
Anti-ddddk, Anti-dykddddk
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Species reactivity:
-
Application:
—
Citations:
32
conjugate
agarose conjugate
antibody product type
primary antibodies
form
suspension
isotype
IgG12b
capacity
≥0.6 mg/mL, gel binding capacity
storage temp.
−20°C
Quality Level
General description
ANTI-FLAG® M1 Agarose Affinity Gel is a purified IgG2B monoclonal antibody covalently attached to agarose.
Application
For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.
Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.
immunoprecipitation (IP): suitable
Elution - FLAG peptide, Glycine, pH 3.5 EDTA
Learn more product details in our FLAG® application portal.
Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.
immunoprecipitation (IP): suitable
Elution - FLAG peptide, Glycine, pH 3.5 EDTA
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Features and Benefits
- Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
- Fusion protein may be eluted from affinity resin by mild elution with EDTA.
- A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.
Physical form
Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide
Other Notes
ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Ai Lu et al.
Biochemical and biophysical research communications, 513(3), 746-752 (2019-04-17)
Phosphoribosylformylglycinamidine synthase (PFAS) is an essential enzyme in de novo synthesis of purine. Previously, PFAS has been reported to modulate RIG-I activation during viral infection via deamidation. In this study, we sought to identify potential substrates that PFAS can deamidate.
Weinan Zheng et al.
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Carol S Bookwalter et al.
The Journal of biological chemistry, 292(47), 19290-19303 (2017-10-06)
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Oncotarget, 10(46), 4743-4760 (2019-08-16)
Triple-negative breast cancer (TNBC) is very aggressive and lacks specific therapeutic targets. Ribosome RNAs (rRNAs) are central components of ribosomes and transcribed in nucleoli, and the level of rRNA transcription greatly affects ribosome production and cell proliferation. We have reported
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Molecular cell, 68(4), 698-714 (2017-11-18)
Telomere elongation through telomerase enables chromosome survival during cellular proliferation. The conserved multifunctional shelterin complex associates with telomeres to coordinate multiple telomere activities, including telomere elongation by telomerase. Similar to the human shelterin, fission yeast shelterin is composed of telomeric
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이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합형 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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