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Merck

A5228

Anti-α-Smooth Muscle Actin (ACTA2) Antibody,

mouse monoclonal, 1A4

동의어(들):

Monoclonal Anti-Actin, α-Smooth Muscle, Acta2 Antibody, Alpha Smooth Muscle Actin Antibody Sigma, SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, Sma Antibody, alpha SMA, SMA

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
1A4, monoclonal
Application:
ARR, ELISA (i), ICC, IF, IHC (p), WB
Species reactivity:
human, mouse, rat, chicken, frog, canine, guinea pig, rabbit, bovine, goat, sheep, snake
Citations:
794
Technique(s):
immunocytochemistry: suitable using smooth muscle cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, indirect ELISA: suitable, indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections, microarray: suitable, western blot: suitable using smooth muscle cells
Uniprot accession no.:
P62736

주요 문서

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제품 이름

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, clone 1A4, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

PBS solution

mol wt

antigen ~42 kDa

species reactivity

human, mouse, rat, chicken, frog, canine, guinea pig, rabbit, bovine, goat, sheep, snake

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable using smooth muscle cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, indirect ELISA: suitable, indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections, microarray: suitable, western blot: suitable using smooth muscle cells

isotype

IgG2a

UniProt accession no.

P62736

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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Application

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal has been used in:
  • immunofluorescence staining
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biochem/physiol Actions

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, cytokinesis, secretion, cytoplasmic streaming and phagocytosis. It has been shown that the relative proportion of actin isoforms are different in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the NH2 terminal synthetic decapeptide of a smooth muscle actin, coupled to keyhole limpet hemocyanin (KLH).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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시험 성적서(COA)

Lot/Batch Number
109M4845V
22190719
029M4807V
22181214
128M4779V

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I
Kern S, et al.
FEBS Open Bio, 4(8), 11-17 (2014)
Special characteristics of culturing mature human bladder smooth muscle cells on human amniotic membrane as a suitable matrix
Sharifiaghdas F, et al.
Urology, 6(4), 283-288 (2009)
Risk of dissection in thoracic aneurysms associated with mutations of smooth muscle alpha-actin 2 (ACTA2)
Disabella E, et al.
Heart, 97(4), 321-326 (2011)
Jennifer J P Collins et al.
American journal of physiology. Lung cellular and molecular physiology, 299(6), L852-L860 (2010-10-12)
Bronchopulmonary dysplasia is associated with chorioamnionitis and fetal lung inflammation. Ureaplasma species are the bacteria most frequently isolated from chorioamnionitis. Very chronic ureaplasma colonization of amniotic fluid causes low-grade lung inflammation and functional lung maturation in fetal sheep. Less is
Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
Ning H, et al.
Differentiation, 74(9-10), 510-518 (2006)
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Data Sheet - A5228
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