C8487
Anti-Caspase 3, Active antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
동의어(들):
Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
115
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, bovine, human, pig, canine, mouse
packaging
antibody small pack of 25 μL
technique(s)
indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine, microarray: suitable, western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)
General description
Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.
Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.
Immunogen
Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.
Application
Anti-Caspase 3, Active antibody has been used:
- for western blotting of cytochrome c for caspase activation
- as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
- in Western blot analysis of activated caspase 3
- as a primary antibody in immunodetection of rat brain sections
Biochem/physiol Actions
Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.
Physical form
Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Ralf A Linker et al.
Brain : a journal of neurology, 134(Pt 3), 678-692 (2011-03-01)
Inflammation and oxidative stress are thought to promote tissue damage in multiple sclerosis. Thus, novel therapeutics enhancing cellular resistance to free radicals could prove useful for multiple sclerosis treatment. BG00012 is an oral formulation of dimethylfumarate. In a phase II
Catherine Coirault et al.
American journal of physiology. Heart and circulatory physiology, 292(2), H1009-H1017 (2006-10-17)
Intrinsic muscle abnormalities affecting skeletal muscle are often reported during chronic heart failure (CHF). Because myosin is the molecular motor of force generation, we sought to determine whether its dysfunction contributes to skeletal muscle weakness in CHF and, if so
Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma
Liu PF, et al.
Testing, 12(7) (2017)
Caspases: an apoptosis mediator
Palai T K and Mishra S R
Journal of Advanced Veterinary and Animal Research, 2(1) (2015)
Stéphane Pillet et al.
Journal of virology, 83(12), 6279-6287 (2009-04-10)
Morbillivirus infections are characterized by severe leukopenia and immune suppression that develop even before the onset of clinical signs. To characterize in more detail the fate of the immune cells during the critical first week, we evaluated the overall viability
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