D3035
Deoxyribonucleic acid from human placenta
buffered aqueous solution, sexed, female
동의어(들):
DNA
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Biological source:
human placenta
Form:
buffered aqueous solution
Storage temp.:
−20°C
SMILES string
[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O
InChI
1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
InChI key
AWBASQCACWFTGD-UHFFFAOYSA-N
biological source
human placenta
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.
Application
Deoxyribonucleic acid from human placenta has been used:
- to compare the efficiency of DNA quantification methods
- as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
- as an internal control to estimate the degree of fragmentation of circulating DNA
- for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
- to compare the 70-bp P5 exon sequence between DNA of different human and primate species
- to calculate copy number of genes, relative to normal human tissue
- in PCR reactions
For use in Southern hybridizations.
Other Notes
Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
J E Collins et al.
Genome research, 7(5), 522-531 (1997-05-01)
A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived
Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
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