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Merck

D5905

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

동의어(들):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
NACRES:
NA.83
PubChem Substance ID:
24893981
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:
MFCD00012969
Beilstein/REAXYS Number:
1212988

주요 문서

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제품 이름

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

Quality Level

200

관련 카테고리

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
SLCF6662
SLCD1660
SLBS4779
SLBQ0700V
SLBN2836V

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문서

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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