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Merck

F5803

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

동의어(들):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C14H16N2O2 · 2HCl
CAS 번호:
Molecular Weight:
317.21
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
243-737-5
Beilstein/REAXYS Number:
3917996
MDL number:
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Quality Level

form

crystalline

pH range

6.95-7.05

mp

268 °C

solubility

water: 2.5 mg/mL, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

General description

A preweighed vial containing 50 mg o-Dianisidine.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Disclaimer

Probably carcinogenic.


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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges



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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문


프로토콜

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.