G2150
L-Glutamine solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
동의어(들):
L-Glutamine for cell culture
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
grade
Hybri-Max™
sterility
sterile-filtered
assay
≥99% (TLC)
form
solution
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
color
clear colorless
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
rat ... Ggt1(116568)
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General description
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Application
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Preparation Note
Prepared in cell culture grade water.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
저장 등급
12 - Non Combustible Liquids
Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
A U Lekmanov et al.
Anesteziologiia i reanimatologiia, (1)(1), 49-51 (2013-07-03)
Comparing analysis of randomized study was conducted. 40 Pediatric patients (2-15 years old) with heavy thermic burns and concomitant injuries were recruited into the study to assess the glutamine solution intravenous use efficiency. Parenteral feeding and feeding with glutamine solution
Ethan B Butler et al.
Cancer research, 73(9), 2709-2717 (2013-04-24)
Cancer cells are markedly different from normal cells with regards to how their metabolic pathways are used to fuel cellular growth and survival. Two basic metabolites that exemplify these differences through increased uptake and altered metabolic usage are glucose and
문서
Importance and uses of glutamine in hybridoma and mammalian cell culture
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