G7877
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type VII, ammonium sulfate suspension, ≥200 units/mg protein
동의어(들):
glucose-6-phosphate dehydrogenase (NADP+), G-6-P-DH, Zwischenferment
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
≥200 units/mg protein
Biological source:
bakers yeast
biological source
bakers yeast
Quality Level
type
Type VII
form
ammonium sulfate suspension
specific activity
≥200 units/mg protein
mol wt
128 kDa
technique(s)
cell based assay: suitable
solubility
water: soluble
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Gene Information
Saccharomyces cerevisiae ... ZWF1(855480)
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General description
Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).
Application
Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 7.0
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
D Janowski et al.
Theriogenology, 78(3), 656-669 (2012-05-15)
The close contact and interaction between the oocyte and the follicular environment influence the establishment of oocyte developmental competence. Moreover, it is assumed that apoptosis in the follicular cells has a beneficial influence on the developmental competence of oocytes. The
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
Johannes Yayo et al.
Applied and environmental microbiology, 87(9) (2021-02-21)
The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would
Nanette G Vrijenhoek et al.
ALTEX, 39(2), 207–220-207–220 (2022-01-19)
Chemical read-across is commonly evaluated without specific knowledge of the biological mechanisms leading to observed adverse outcomes in vivo. Integrating data that indicate shared modes of action in humans will strengthen read-across cases. Here we studied transcriptomic responses of primary
문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
프로토콜
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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