H1029
Monoclonal Anti-polyHistidine antibody produced in mouse
clone HIS-1, ascites fluid
동의어(들):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HIS-1, monoclonal
Application:
DB, ELISA (i), IP, WB
Citations:
331
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
liquid
technique(s)
dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.
Biochem/physiol Actions
The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical form
Suppled as ascites fluid with 15mM sodium azide.
Other Notes
Recommended dilution for immunoblotting is 1:3000
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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저장 등급
10 - Combustible liquids
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Prokunina-Olsson L, et al.
Nature Genetics, 45(2), 164-164 (2013)
G protein-coupled receptor-promoted trafficking of G?1?2 leads to AKT activation at endosomes via a mechanism mediated by G?1?2-Rab11a interaction.
Garcia-Regalado A, et al.
Molecular Biology of the Cell, 19(10), 4188-4200 (2008)
Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Savitsky D A, et al.
Proceedings of the National Academy of Sciences of the USA (2010)
Elise Pinta et al.
The Journal of biological chemistry, 285(36), 28333-28342 (2010-07-03)
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Association of a novel pre-ribosomal complex in Trypanosoma brucei.
Wang L
Eukaryotic Cell (2013)
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