H3506

Hyaluronidase from bovine testes

Name: Hyaluronidase from bovine testes
Brand: SIGMA

Type I-S, lyophilized powder, 400-1000 units/mg solid

동의어(들):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-464-3

MDL number:

MFCD00131351

EC 번호:

3.2.1.35 (BRENDA, IUBMB)

Specific activity:

400-1000 units/mg solid

주요 문서

모든 문서 보기

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

type

Type I-S

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

유사한 제품을 찾으십니까? 방문 제품 비교 안내

Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Laser-ultrasonic delivery of agents into articular cartilage.

Heikki J Nieminen et al.

Scientific reports, 7(1), 3991-3991 (2017-06-24)

Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound

Lubricin binds cartilage proteins, cartilage oligomeric matrix protein, fibronectin and collagen II at the cartilage surface.

Sarah A Flowers et al.

Scientific reports, 7(1), 13149-13149 (2017-10-17)

Lubricin, a heavily O-glycosylated protein, is essential for boundary lubrication of articular cartilage. Strong surface adherence of lubricin is required given the extreme force it must withstand. Disulfide bound complexes of lubricin and cartilage oligomeric matrix protein (COMP) have recently

Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential.

Kawase Y et al.

Reproduction (Cambridge, England), 133, 841-841 (2007)

A SAGE (serial analysis of gene expression) view of breast tumor progression.

Porter DA et al.

Cancer Research, 61, 5697-5697 (2001)

Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus.

Kirshenbaum LA et al.

The Journal of Clinical Investigation, 92, 381-381 (1993)

모든 관련된 서류 보기

문서

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

프로토콜

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

type

form

specific activity

mol wt

technique(s)

UniProt accession no.

application(s)

shipped in

storage temp.

Quality Level

관련 카테고리

설명

Application

Biochem/physiol Actions

Other Notes

안전성 정보

pictograms

signalword

hcodes

pcodes

Hazard Classifications

저장 등급

wgk

flash_point_f

flash_point_c

ppe

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

관련 참고 프로토콜

문서

프로토콜

관련 콘텐츠

기술 서비스