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Merck

H3757

Hyaluronidase from bovine testes

Type VIII, lyophilized powder, 300-1,000 U/mg

동의어(들):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC 번호:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
300-1,000 U/mg
Biological source:
bovine testis

주요 문서

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도움 문의

biological source

bovine testis

conjugate

conjugate (Glucosaminoglycan)

type

Type VIII

form

lyophilized powder

specific activity

300-1,000 U/mg

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985, Q8SQG8

relevant disease(s)

cancer

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

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관련 카테고리

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
SLCK7710
SLCG7032
SLBZ6642
SLBX1082
SLBT8168

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문서 라이브러리 방문

Kohtaro Miyazawa et al.
Histochemistry and cell biology, 133(1), 125-134 (2009-10-16)
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and
Pin-Yao Lin et al.
PloS one, 14(11), e0224919-e0224919 (2019-11-19)
Unexpected sperm retrieval failure on the day of oocyte retrieval is not common but frequently happened in patients with severe oligospermia or non-obstructive azoospermia(NOA). Oocyte cryopreservation is a common strategy after failed collection of sperm when concurrent ovarian stimulation is
C Rollo et al.
Reproduction (Cambridge, England), 154(4), 375-385 (2017-09-08)
Acetylation of histone proteins is a major determinant of chromatin structure and function. Fertilisation triggers a round of chromatin remodelling that prepares the genome for the first round of transcription from the new embryonic genome. In this study we confirm
Takayuki Yamochi et al.
Journal of assisted reproduction and genetics, 38(10), 2591-2599 (2021-05-11)
To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. When OGCs
Nathan V Welham et al.
Journal of speech, language, and hearing research : JSLHR, 52(4), 1008-1020 (2009-07-31)
To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, and vocal fold vibratory changes resulting from vocal fold scar. Twenty-four 4-month-old male Sprague-Dawley rats were assigned to 1 of 4 experimental groups: chronic vocal
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문서

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

프로토콜

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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