L0770
Lectin from Pisum sativum (pea)
FITC conjugate, lyophilized powder
동의어(들):
Pisum sativum agglutinin, PSA
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
conjugate
FITC conjugate
Quality Level
form
lyophilized powder
potency
<20 μg per mL agglutination activity
composition
Protein, ≥95% biuret
extent of labeling
2-4 mol FITC per mol protein (Lowry).
impurities
salt, free
storage temp.
−20°C
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General description
Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.
Application
Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns
Biochem/physiol Actions
Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Crypticity and functional distribution of the membrane associated ?-L-fucosidase of human sperm
Venditti JJ, et al.
Molecular Reproduction and Development, 74(6), 758-766 (2007)
Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria
Purish LM, et al.
Applied Biochemistry and Microbiology, 49(5), 458-463 (2013)
Amar K Chandra et al.
Environmental toxicology, 32(6), 1823-1835 (2017-02-17)
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress
In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
Tácia Gomes Bergstein-Galan et al.
Theriogenology, 96, 69-75 (2017-05-24)
This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis.
문서
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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