M0650
Medium 199
With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
동의어(들):
Cell growth medium, M199 Medium, TCM 199
제품 이름
Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
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General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture human mesothelial cells
- as a component of the holding medium for the collection of embryos from pluriparous Boer goat
- in the preparation of spheroid-embedded collagen matrices
Preparation Note
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Elena García-Gareta et al.
BioResearch open access, 2(6), 412-420 (2014-01-01)
The clinical demand for effective dermal substitutes continues as current commercially available products present limitations. However, there are no definitive in vitro methods to predict in vivo outcomes such as integration, cellularization and contraction, which may help the development of
Technical Note: Transfer of caprine blastocysts vitrified by the open pulled straw (OPS) or the solid surface procedure and warmed in sucrose-free medium
Garza D, et al.
Small Ruminant Research, 165, 111-114 (2018)
Chiemi Toyama-Mori et al.
Cryobiology, 88, 92-97 (2019-03-14)
Effects of additional physical treatments during vitrification of the bovine ovarian tissue were examined for increasing of permeability of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). The concentrations of EG and Me2SO and histological changes in the ovarian tissue were
Johanna Kohlhaas et al.
Experimental cell research, 399(2), 112446-112446 (2021-01-11)
Communication of vascular cells is essential for the control of organotypic functions of blood vessels. In this context, vascular endothelial cells (EC) act as potent regulators of vascular smooth muscle cell (VSMC) functions such as contraction and relaxation. However, the
Klaus Kratochwill et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 27(3), 937-946 (2011-08-23)
Exposure of mesothelial cells to peritoneal dialysis fluids (PDF) results in cytoprotective cellular stress responses (CSR) that counteract PDF-induced damage. In this study, we tested the hypothesis that the CSR may be inadequate in relevant models of peritoneal dialysis (PD)
문서
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
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