M1567
Malic Dehydrogenase from porcine heart
≥600 units/mg protein (biuret), ammonium sulfate suspension
동의어(들):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
Specific activity:
≥600 units/mg protein (biuret)
General description
Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.
Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Application
Malic Dehydrogenase from porcine heart has been used:
- in qualitative protein binding measurements
- to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
- to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase
Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.
Biochem/physiol Actions
Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.
Physical form
Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Yassir M Al-Mulla Hummadi et al.
Experimental parasitology, 112(2), 85-91 (2005-11-09)
S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets
Heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid
Lowenthal, A., et al.
Journal of Neurochemistry, 7, 135-140 (2006)
Electrochemical Coupled-Enzyme Assays at Carbon Nanotubes
Zhang M, et al.
Analytical Chemistry, 86(18), 9330-9334 (2014)
Malate dehydrogenases-structure and function
Minarik P, et al.
General Physiology and Biophysics, 21(3), 257-266 (2002)
Sensing NADH conformation using phasor analysis on fluorescence spectra
Palo D, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 186(3), 105-111 (2017)
문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
프로토콜
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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