M2154
Medium 199
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
M199 Medium, TCM 199
제품 이름
Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.0-7.6 (25 °C, 209 g/L)
components
sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture rumen epithelial cells from yearling Speckle Park beef heifers
- as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
- as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)
Preparation Note
Supplement with 0.1 g/L L-glutamine.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Melisa Gomez-Velazquez et al.
PLoS genetics, 13(8), e1006985-e1006985 (2017-08-29)
Cardiac progenitors are specified early in development and progressively differentiate and mature into fully functional cardiomyocytes. This process is controlled by an extensively studied transcriptional program. However, the regulatory events coordinating the progression of such program from development to maturation
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
María Isabel Hernández-Alvarez et al.
Cell, 177(4), 881-895 (2019-05-06)
Non-alcoholic fatty liver is the most common liver disease worldwide. Here, we show that the mitochondrial protein mitofusin 2 (Mfn2) protects against liver disease. Reduced Mfn2 expression was detected in liver biopsies from patients with non-alcoholic steatohepatitis (NASH). Moreover, reduced Mfn2
Nigel J Brand et al.
Methods in molecular biology (Clifton, N.J.), 633, 113-124 (2010-03-06)
We describe a method of isolating and maintaining primary cultures of mouse neonatal cardiac myocytes (NCM). This is derived from the well-established procedure for making NCM cultures from rat neonates by sequential digestion of rat ventricular myocardial pieces using a
Martyna Pakuła et al.
International journal of molecular sciences, 20(1) (2019-01-06)
The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT
문서
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
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