Neutralization Solution B

Name: Neutralization Solution B
Brand: SIGMA

동의어(들):

DNA neutralization solution, neutralization buffer DNA extraction

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크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.25

UNSPSC Code:

12161703

기술 서비스

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도움 문의

속성

grade

Molecular Biology

Quality Level

200

form

liquid

feature

Direct PCR

technique(s)

DNA amplification: suitable, DNA extraction: suitable, PCR: suitable

input

crude DNA

storage temp.

−20°C

설명

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp^™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp^™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp^™ PCR Reaction Mix.