N5288
Anti-NQO1 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
동의어(들):
Anti-DIA4, Anti-DT-diaphorase, Anti-Diaphorase 4, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
27
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~28 kDa
species reactivity
mouse, rat, human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells, indirect immunofluorescence: 2-5 μg/mL using human HeLa cells, western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells, western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NQO1(1728)
mouse ... Nqo1(18104)
rat ... Nqo1(24314)
General description
NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors.
NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed.
Immunogen
Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
Application
Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa).
Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:
- western blotting
- immunoprecipitation
- immunofluorescence
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting.
NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
A mutually inhibitory feedback loop between the 20S proteasome and its regulator, NQO1
Moscovitz O, et al.
Molecular Cell, 47, 76-86 (2012)
NAD(P)H:quinone oxidoreductase 1: role as a superoxide scavenger.
Siegel, D., et al.
J. Mol. Pharmacol., 65(5), 1238-1247 (2004)
NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphisms
Ross D, et al.
Chemico-Biological Interactions, 77-97 (2000)
Annette M Krais et al.
Archives of toxicology, 90(4), 839-851 (2015-05-23)
The tumour suppressor p53 is one of the most important cancer genes. Previous findings have shown that p53 expression can influence DNA adduct formation of the environmental carcinogen benzo[a]pyrene (BaP) in human cells, indicating a role for p53 in the
Christoph Otto et al.
BMC complementary and alternative medicine, 16, 160-160 (2016-06-02)
Fermented wheat germ extract (FWGE) sold under the trade name Avemar exhibits anticancer activity in vitro and in vivo. Its mechanisms of action are divided into antiproliferative and antimetabolic effects. Its influcence on cancer cell metabolism needs further investigation. One
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