N6658
Nutrient Mixture F-12 Ham
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
동의어(들):
Ham’s F-12
제품 이름
Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.
Application
Nutrient Mixture F-12 Ham has been used:
- to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
- to culture LAN-1 cells
- to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
- with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lilla Krokker et al.
Pathology oncology research : POR, 27, 640676-640676 (2021-07-15)
In vitro monolayer conditions are not able to reproduce the complexity of solid tumors, still, there is scarce information about the 3D cell culture models of endocrine tumor types. Therefore, our aim was to develop in vitro 3D tumor models
Kazuo Takazawa et al.
The Kobe journal of medical sciences, 54(1), E14-E22 (2008-09-06)
Insulin induces translocation of the glucose transporter GLUT4 from intracellular storage compartment to the plasma membrane via complex mechanisms that require intact cytoskeletal networks. In cultured adipocytes, conventional kinesin motor proteins have been proposed to mediate GLUT4 movements on microtubules.
Yoshinobu Takahashi et al.
Oncology letters, 14(1), 264-270 (2017-07-12)
Whether the poor prognosis of primary central nervous system lymphoma (PCNSL) compared with systemic diffuse large B cell lymphoma (DLBCL) is attributable to the immune privilege of the intracerebral location or to intrinsic differences in the biological characteristics of two
Toshimichi Tanaka et al.
Annals of surgical oncology, 26(5), 1401-1411 (2019-02-02)
Treatment-resistance genes limiting anticancer therapy have not been well clarified in colorectal cancer (CRC). We explored gene expression profiles to identify biomarkers for predicting treatment resistance to an anticancer drug in CRC. Six CRC cell lines were treated with phenylbutyrate
Jianjia Fan et al.
Journal of lipid research, 59(5), 830-842 (2018-03-23)
apoE is the primary lipid carrier within the CNS and the strongest genetic risk factor for late onset Alzheimer's disease (AD). apoE is primarily lipidated via ABCA1, and both are under transcriptional regulation by the nuclear liver X receptor (LXR).
관련 콘텐츠
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