NUC201
Nuclei Isolation Kit: Nuclei PURE Prep
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
동의어(들):
Sucrose centrifugation nuclei isolation
usage
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
packaging
pkg of 1 kit
storage condition
dry at room temperature
application(s)
cell analysis
foreign activity
nuclease and protease, free
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.
Biochem/physiol Actions
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
키트 구성품 전용
제품 번호
설명
- Nuclei PURE Lysis Buffer 180 mL
signalword
Danger
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
Ju-Chi Liu et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 4503747-4503747 (2021-01-26)
Diabetes is associated with the development of myocardial fibrosis, which is related to various cardiac diseases. Cafestol, one of the active ingredients in coffee, has been reported to exert biological effects. However, whether cafestol can ameliorate diabetes-induced cardiac fibrosis remains
Yin Ying et al.
Frontiers in endocrinology, 9, 774-774 (2019-01-09)
Hyperglycemia induces chronic inflammation and oxidative stress in cardiomyocyte, which are the main pathological changes of diabetic cardiomyopathy (DCM). Treatment aimed at these processes may be beneficial in DCM. Phloretin (PHL), a promising natural product, has many pharmacological activities, such
Nuclei from rat liver: isolation method that combines purity with high yield.
G Blobel et al.
Science (New York, N.Y.), 154(3757), 1662-1665 (1966-12-30)
Wei Chen et al.
The Journal of biological chemistry, 283(24), 16293-16298 (2008-04-10)
Previous studies have shown that the acute stimulation of endothelial nitric-oxide synthase (eNOS) mRNA transcription by laminar shear stress is dependent on nuclear factor kappa B (NFkappaB) subunits p50 and p65 binding to a shear stress response element (SSRE) in
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
문서
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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