O4886
Monoclonal Anti-Opsin antibody produced in mouse
clone RET-P1, ascites fluid
동의어(들):
Anti-Opsin Antibody, Mouse Anti-Opsin, Opsin Detection Antibody
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
RET-P1, monoclonal
Application:
ELISA (i), EM, ICC, IHC (f), RIA, WB
Citations:
57
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
RET-P1, monoclonal
mol wt
antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet)
contains
15 mM sodium azide
species reactivity
goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle
technique(s)
electron microscopy: suitable, immunocytochemistry: suitable using cultured cells, immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye, indirect ELISA: suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)
관련 카테고리
General description
Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.
Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells.
Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.
Immunogen
rat retinal membranes.
Application
Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology.
Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.
Biochem/physiol Actions
Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Wild-type opsin does not aggregate with a misfolded opsin mutant
Gragg M, et al.
Biochimica et Biophysica Acta - Biomembranes, 1858(8), 1850-1859 (2016)
Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit
Hu Y, et al.
Investigative Ophthalmology & Visual Science, 49(7), 3185-3192 (2008)
Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Muller glial cell development
Poche RA, et al.
The Journal of Comparative Neurology, 510(3), 237-250 (2008)
D Hicks et al.
Journal of neurocytology, 15(2), 219-230 (1986-04-01)
Lectin and rhodopsin antibody binding sites were studied in developing and adult rat photoreceptors in order to compare changes in the total carbohydrate pool with the movement of a known glycoprotein rhodopsin. Electron microscope immunocytochemical techniques utilizing modified colloidal gold
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution
Solovei I, et al.
Cell, 137(2), 356-368 (2009)
관련 콘텐츠
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.