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Merck

P4744

Phosphatase substrate

chromogenic, powder

동의어(들):

4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS 번호:
333338-18-4
Molecular Weight:
371.14
NACRES:
NA.83
PubChem Substance ID:
24898495
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
MFCD00066288

주요 문서

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제품 이름

Phosphatase substrate, powder

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

storage temp.

−20°C

Quality Level

200

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관련 카테고리

Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP).

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
SLCG8427
SLBZ6874
SLCC9618
SLBS6000
SLBV3754

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문서

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

프로토콜

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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