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Merck

P8203

PIPES

BioXtra, ≥99% (titration)

동의어(들):

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C8H18N2O6S2
CAS 번호:
5625-37-6
Molecular Weight:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24898917
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:
MFCD00006159

주요 문서

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InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

product line

BioXtra

assay

≥99% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.5% (as SO4)

loss

≤0.5% loss on drying, 110°C

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.2%

cation traces

Al: ≤0.005%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.005%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.1%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.1 at 260 in 1 M NaOH at 0.5 M, ≤0.1 at 280 in 1 M NaOH at 0.5 M

application(s)

diagnostic assay manufacturing

Quality Level

400

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관련 카테고리

Analysis Note

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Other Notes

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

저장 등급

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
SLCL4577
SLCF8825
SLCF1397
SLCC4968
SLBZ9381

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문서 라이브러리 방문

Y Zhou et al.
The Journal of biological chemistry, 275(10), 6975-6979 (2000-03-04)
The poor stability of membrane proteins in detergent solution is one of the main technical barriers to their structural and functional characterization. Here we describe a solution to this problem for diacylglycerol kinase (DGK), an integral membrane protein from Escherichia
Q Yu et al.
Analytical biochemistry, 253(1), 50-56 (1997-11-14)
Of the 20 well-known buffers proposed by Good, all but 3 form metal ion complexes which can result in serious interferences, particularly in protein analyses. The structural features responsible for such complex formation have been identified. Based on a mechanistic
A Giraudel et al.
Biochemistry, 37(24), 8724-8734 (1998-06-24)
The dissociation and separation of the tubulin alpha- and beta-subunits have been achieved by binding alpha-subunits to an immunoadsorbent gel and selectively inducing release of free beta-subunits. The immunoadsorbent gel was prepared by coupling the monoclonal antibody YL1/2 to Sepharose
Sambrook , J. and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 7-7 (2001)
S Haviernick et al.
Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy
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프로토콜

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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