S9626
Sulfatase from Helix pomatia
Type H-1, sulfatase ≥10,000 units/g solid
동의어(들):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-772-1
MDL number:
type
Type H-1
Quality Level
form
powder
sulfatase activity
≥10,000 units/g solid
secondary activity
≥300 unit/mg solid β-glucuronidase (at pH 5.0)
mol wt
~85 kDa by gel filtration
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
Application
Sulfatase from Helix pomatia has been used in:
- sample extraction
- sulfatase assays
- enzymatic hydrolysis
- extract preparation for glucosinolate analysis
Biochem/physiol Actions
Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
저장 등급
13 - Non Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
프로토콜
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)