T0198
Anti-β-Tubulin antibody, Mouse monoclonal
clone D66, purified from hybridoma cell culture
동의어(들):
Anti-Tubulin beta-1 chain
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
D66, monoclonal
Application:
ELISA (i), ICC, IP, WB
Species reactivity:
human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey
Citations:
186
Technique(s):
immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone), immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
D66, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone), immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)
rat ... Tubb2(29212), Tubb2c(296554)
Application
Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models.
Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in immunofluorescence assay and enzyme linked immunosorbent assay (ELISA).
Monoclonal Anti-β-tubulin antibody produced in mouse has been used in western blotting and immunocytochemistry.
Biochem/physiol Actions
Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β- tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
β-Tubulin belongs to the subfamily of tubulins, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β−Tubulin structure is characterized with core of two β -sheets enclosed by a-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (β I (class I), β II (class II), β III (class III), β IVa (class IVa), β IVb (class IVb), β V (class V), and β VI (class VI)).
Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the D66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a sea urchin (Lytechinus pictus) sperm axonemal proteins.
Immunogen
sea urchin (Lytechinus pictus) sperm axonemal proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Expression and subcellular distribution of UNC119a, a protein partner of transducin alpha subunit in rod photoreceptors
Sinha S, et al.
Cellular Signalling, 25(1), 341-348 (2013)
Activity-Dependent Shedding of the NMDA Receptor Glycine Binding Site by Matrix Metalloproteinase 3: A PUTATIVE Mechanism of Postsynaptic Plasticity
Pauly T, et.al.
PLoS ONE, 3, e2681-e2681 (2008)
Yu Tian et al.
Molecular and cellular biology, 27(18), 6383-6395 (2007-07-20)
Studies of a TAZ knockout mouse reveal a novel function of the transcriptional regulator TAZ, that is, as a binding partner of the F-box protein beta-Trcp. TAZ-/- mice develop polycystic kidney disease (PKD) and emphysema. The calcium-permeable cation channel protein
Jun Yang et al.
Frontiers in bioscience (Landmark edition), 17, 1165-1183 (2011-12-29)
Usher syndrome is the most common deafness-blindness caused by genetic mutations. To date, three genes have been identified underlying the most prevalent form of Usher syndrome, the type II form (USH2). The proteins encoded by these genes are demonstrated to
Deirdre C Lyons et al.
Developmental biology, 334(1), 46-58 (2009-07-18)
The unequal division of the CD blastomere at second cleavage is critical in establishing the second embryonic axis in the leech Helobdella, as in other unequally cleaving spiralians. When CD divides, the larger D and smaller C blastomeres arise invariantly
문서
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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