T3405
3,3′,5,5′-Tetramethylbenzidine dihydrochloride
chromogenic, tablet
동의어(들):
4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C16H20N2 · 2HCl
CAS 번호:
Molecular Weight:
313.27
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
264-769-6
MDL number:
제품 이름
3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
InChI key
NYNRGZULARUZCC-UHFFFAOYSA-N
InChI
1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
SMILES string
Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
form
tablet
mp
≥300 °C
storage temp.
2-8°C
Quality Level
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
- anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
- anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
- in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Yan-Qin Huang et al.
Analytical and bioanalytical chemistry, 410(28), 7385-7394 (2018-09-15)
We certify that protamine-gold nanoclusters (PRT-AuNCs) synthesized by one-pot method exhibit peroxidase-like activity. The catalytic activity of PRT-AuNCs followed typical Michaelis-Menten kinetics and exhibited higher affinity to 3,3',5,5'-tetramethylbenzidine (TMB) as the substrate compared to that of natural horseradish peroxidase. Meanwhile
Beth C Holbrook et al.
Virology, 476, 124-133 (2014-12-30)
Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is
Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach
Troquier L, et al.
Current Alzheimer Research, 9(4), 397-405 (2012)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
Thiru Vanniasinkam et al.
Methods in molecular biology (Clifton, N.J.), 1785, 121-128 (2018-05-02)
This chapter describes a strategy for mapping linear B-cell epitopes of proteins using synthetic biotinylated peptides in an ELISA.A set of overlapping peptides were designed based upon a known amino acid sequence of the target protein, VapA (Virulence-associated Protein A)
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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