T7451
Anti-Acetylated Tubulin antibody, Mouse monoclonal
clone 6-11B-1, purified from hybridoma cell culture
동의어(들):
Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Anti Acetyl Tubulin antibody, Anti Acetyl-alpha tubulin
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
6-11B-1, monoclonal
Application:
DB, EM, IHC, RIA, WB
Citations:
836
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6-11B-1, monoclonal
form
buffered aqueous solution
species reactivity
bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
dot blot: suitable, electron microscopy: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: 0.03-0.06 μg/mL using total rat brain extract
isotype
IgG2b
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
acetylation (Lys40)
Quality Level
Gene Information
human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
General description
α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.
Immunogen
acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).
Application
Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:
- quantitative dot blot
- immunofluorescence
- Western blot
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
- solid-phase radioimmunoassay (RIA)
- electron microscopy
Biochem/physiol Actions
Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.
The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders
Giles RH, et al.
Nature Protocols, 9(12), 2725-2725 (2014)
M LeDizet et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5720-5724 (1987-08-01)
An acetylation site of Chlamydomonas axonemal alpha-tubulins was identified near, or within, the binding site of 6-11B-1, a monoclonal antibody specific for posttranslationally acetylated alpha-tubulins. In a first approach, axonemal proteins were hydrolyzed by formic acid, cyanogen bromide, or chymotrypsin
All tubulins are not alike: heterodimer dissociation differs among different biological sources
Montecinos-Franjola F, et al.
The Journal of biological chemistry, 9(12), jbc-RA119 (2019)
Basal cells as stem cells of the mouse trachea and human airway epithelium.
Rock JR
Proceedings of the National Academy of Sciences of the USA, 106(31), 12771-12775 (2009)
G Piperno et al.
The Journal of cell biology, 104(2), 289-302 (1987-02-01)
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy
관련 콘텐츠
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.