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Merck

T9784

Tricine

BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)

동의어(들):

N-[Tris(hydroxymethyl)methyl]glycine

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS 번호:
5704-04-1
Molecular Weight:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24900603
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:
MFCD00004277

주요 문서

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InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

SMILES string

OCC(CO)(CO)NCC(O)=O

product line

BioXtra

assay

≥99% (titration)

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

4.0-6.0 (1 M in H2O)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.1%, sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 1 M, ≤0.1 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

Quality Level

400

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관련 카테고리

General description

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Application

Buffer component for separation of low molecular weight peptides.
Tricine has been used:
  • in a polymerase chain reaction (PCR) buffer used in the single-cell collection
  • as a component of lactotroph feeding medium for culturing lactotrophs
  • to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

저장 등급

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
SLCJ5840
SLCJ0569
SLCJ0442
SLCH8687
SLCC3522

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Encyclopedia of Dairy Sciences (2011)
Joke Mertens et al.
Bio-protocol, 9(13), e3283-e3283 (2019-07-05)
Detecting heteroplasmies in the mitochondrial DNA (mtDNA) has been a challenge for many years. In the past, Sanger sequencing was the main option to perform this analysis, however, this method could not detect low frequency heteroplasmies. Massive Parallel Sequencing (MPS)
Analysis of gene promoter regulation by tumor suppressor genes.
Kumaravel Somasundaram et al.
Methods in molecular biology (Clifton, N.J.), 223, 101-116 (2003-06-05)
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
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