V4505
Anti-Vinculin Antibody
mouse monoclonal, VIN-11-5
동의어(들):
Vinculin Antibody Sigma, Anti-VCL
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
VIN-11-5, monoclonal
Application:
ARR, IF, WB
Citations:
236
제품 이름
Monoclonal Anti-Vinculin antibody produced in mouse, clone VIN-11-5, ascites fluid
storage temp.
−20°C
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
VIN-11-5, monoclonal
mol wt
antigen 116 kDa
species reactivity
bovine, human, chicken, mouse
technique(s)
indirect immunofluorescence: 1:50 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
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General description
Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.
Immunogen
smooth muscle vinculin from chicken gizzard.
Application
Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
- western blotting.
- immunofluorescence.
- immunocytochemistry.
Biochem/physiol Actions
Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.
The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.
Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Julia Fröbel et al.
Molecular & cellular proteomics : MCP, 12(5), 1272-1280 (2013-02-06)
Bleeding complications are a significant clinical problem in patients with myelodysplastic syndromes even at sufficient platelet counts (>50,000/μl). However, the underlying pathology of this hemorrhagic diathesis is still unknown. Here, we analyzed the platelet proteome of patients with myelodysplastic syndromes
W Xu et al.
Journal of cell science, 111 ( Pt 11), 1535-1544 (1998-05-15)
Vinculin plays a role in signaling between integrins and the actin cytoskeleton. We reported earlier that F9-derived cells lacking vinculin are less spread, less adhesive, and move two times faster than wild-type F9 cells. Expression of intact vinculin in null
Three-dimensional structure of vinculin bound to actin filaments
Janssen MEW, et al.
Molecular Cell, 21(2), 271-281 (2006)
E P Moiseyeva et al.
The Journal of biological chemistry, 268(6), 4318-4325 (1993-02-25)
The human vinculin gene contains 22 exons ranging in size from 71 base pairs (bp) to 303 bp (average 155 bp) with the exception of exon 22 which contains 144 bp of coding sequence and 1848 bp of 3'-untranslated sequence
Leonid Schneider
PloS one, 9(1), e87228-e87228 (2014-01-30)
We recently reported that neural stem cells (NSCs) become senescent and commit to astrocytic differentiation upon X-ray irradiation. Surprisingly, under self-renewing culture conditions, some of these senescent cells undergo p53-independent apoptosis, which can be suppressed by caspase inhibition and BCL2
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