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Merck

Y04C-02-5PK

CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Synonym(s):

Haploid Yeast Cell Plate, Haploid Yeast Plate, ONIX Yeast Plate

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202

Key Documents

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Product Name

CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron), The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

material

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized 3.5–5.0 μm

shipped in

ambient

Related Categories

Application

CellASIC® ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) has been used in live-cell microscopy and analysis of Schizosaccharomyces pombe cells. It has also been used in live cell imagining of Saccharomyces cerevisiae strains. This CellASIC ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) is also suitable for:
  • Time-lapse analysis of yeast cells
  • Long-term continuous perfusion experiments
  • Solution exchange experiments (induction, inhibition, drug dosing, etc.)
  • Comparison of up to 4 different cell types or exposure conditions(media components) in parallel
  • Cell division tracking (follow mother/daughter cells over generations)
  • Temperature and gas atmospheric control (temperature shift, anoxic conditions etc.)

Features and Benefits

  • Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
  • Enables perfusion-based, long-term, live-cell analysis with solutionswitching
  • The plate offers a controlled and dynamicmicroenvironment for cells

General description

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining the x,y position over time.

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ping Wei et al.
Nature, 488(7411), 384-388 (2012-07-24)
Bacterial pathogens have evolved specific effector proteins that, by interfacing with host kinase signalling pathways, provide a mechanism to evade immune responses during infection. Although these effectors contribute to pathogen virulence, we realized that they might also serve as valuable
A Mammalian-like DNA damage response of fission yeast to nucleoside analogs.
Sabatinos SA, Mastro TL, Green MD, Forsburg SL
Genetics, 193 (1), 143-157 (2013)
Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.
Clara Bermejo, Farzad Haerizadeh, Hitomi Takanaga, Diane Chermak, Wolf Frommer
Nature Protocols, 6, 1806-1817 (2011)
Commitment to a cellular transition precedes genome-wide trasncriptional change.
Doncic A, Falleur-Fettig M, Skotheim J
Molecular Cell, Vol. 43, Issue 4, 515-527 (2011)
Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.
Sabatinos SA, Green MD, Forsburg SL
Molecular and cellular biology, 32 (24), 4986-4997 (2012)
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Related Content

Demonstration of long-term culture and in-plate staining protocols using the CellASIC(R) ONIX Microfluidic platform

The CellASIC™ ONIX Microfluidic System, in conjunction with the M04S microfluidic plate, provides perfusion-based microenvironment control for long-term, high quality, live cell microscopy. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health. Four upstream fluidic channels allow controlled exposure of the cells to different solutions during live imaging. The plate can also be cultured in a standard incubator using a dedicated gravity driven flow channel. The cells are in contact with a #1.5 thickness (170 µm) optical glass surface, enabling high quality imaging using an inverted microscope. An integrated micro-incubator system delivers temperature and gas control to the microfluidic chambers.

Microincubator for Long-Term, Live Cell Microscopy and Hypoxic Culture

Microincubator for Long-Term, Live Cell Microscopy and Hypoxic Culture

Live cell imaging of yeast with the CellASIC®platform reveals effect of the extracellular milieu
Bacteria Publications Using the CellASIC®ONIX Microfluidic System
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Global Trade Item Number

SKUGTIN
Y04C-02-5PK04053252823633

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