usage
kit sufficient for 50 reactions
analyte chemical class(es)
proteins (FLAG® Affinity Gels)
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Application
Learn more product details in our FLAG® application portal.
Features and Benefits
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3xFLAG™ peptide provides efficient and gentle elution through direct competition
Other Notes
All prodedures to be performed at 2-8°C.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
10 - Combustible liquids
wgk
WGK 3
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
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