MITOISO2

Mitochondria Isolation Kit

Name: Mitochondria Isolation Kit
Brand: SIGMA

sufficient for 50 applications (2-5 x 10⁷cells), isolation of enriched mitochondrial fraction from cells

동의어(들):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.32

UNSPSC Code:

12352200

주요 문서

모든 문서 보기

기술 서비스

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도움 문의

기술 서비스

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도움 문의

usage

sufficient for 50 applications (2-5 x 10⁷cells)

technique(s)

ELISA: suitable, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:

Ewing′s Sarcoma Family of Tumor (ESFT) cells
blood samples of tuberculosis patients
human neuroblastoma cells and glioma cells
myotubes
HepG2 cells

Features and Benefits

The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
The isolated mitochondria will mostly have intact inner and outer membranes.
Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

This product is for R&D use only, not for drug, household, or other uses.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Use ultrapure water for the preparation of reagents.
Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

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제품 번호

설명

SDS

C2978CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS

P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS

C0356Protein Extraction Reagent Type 4 1 bottleSDS

pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302,H315,H319,H351,H361fd,H411

pcodes

P202 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

저장 등급

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation and Cellular Bioenergetics in Human Down Syndrome Fibroblasts.

Theodora Panagaki et al.

Biomolecules, 10(4) (2020-04-29)

Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate

Mitochondrial outer membrane permeability change and hypersensitivity to digitonin early in staurosporine-induced apoptosis.

Shili Duan et al.

The Journal of biological chemistry, 278(2), 1346-1353 (2002-10-31)

We have shown here that the apoptosis inducer staurosporine causes an early decrease in the endogenous respiration rate in intact 143B.TK(-) cells. On the other hand, the activity of cytochrome c oxidase is unchanged for the first 8 h after

The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain

Fossati S, et al.

Neurobiology of Disease, 86, 29-40 (2016)

Activation of murine microglial cell lines by lipopolysaccharide and interferon-gamma causes NO-mediated decreases in mitochondrial and cellular function.

D W Moss et al.

The European journal of neuroscience, 13(3), 529-538 (2001-02-13)

Activation of murine microglial and macrophage cell lines with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) resulted in the induction of the inducible form of nitric oxide synthase (NOS) and the release of micromolar amounts of NO into the surrounding medium. The

Synthetic galectin-3 inhibitor increases metastatic cancer cell sensitivity to taxol-induced apoptosis in vitro and in vivo.

Vladislav V Glinsky et al.

Neoplasia (New York, N.Y.), 11(9), 901-909 (2009-09-03)

At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin

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문서

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Mitochondria Isolation Kit